Variants from the Diverse Virus Population Identified at Seroconversion of a Clade A Human Immunodeficiency Virus Type 1-Infected Woman Have Distinct Biological Properties

摘要: Development of effective therapeutics to prevent new infections with human immunodeficiency type 1 (HIV-1) is predicated on an understanding the properties that provide a selective advantage transmitted viral population. In contrast homogeneous virus population typifies early HIV-1 infection men, in women recently infected clade A genetically diverse, based evaluation envelope gene. longitudinal study evolution several suggested representative variants detected at seroconversion had distinct biological affected fitness. To test this hypothesis, full-length, infectious molecular clone, Q23-17, was obtained from woman year following seroconversion, and chimeric viruses containing genes 27-month-postseroconversion populations were constructed. Dendritic cells (DC) could transfer variant Q23ScA, which dominated closely related 1-year isolate Q23-17 resting peripheral blood mononuclear (PBMC). contrast, PBMC exposed DC pulsed Q23ScB, infrequently samples after or 27-month chimeras inconsistently infected. Additionally, quiescent Q23ScA proliferated more robustly than uninfected other response immobilized anti-CD3. Stimulation tetanus toxoid led increased proportion CD45RA+ decreased expression CD28 CD45RO+ cultures Q23-17-infected PBMC. These data demonstrate heterogeneous Kenyan have features may influence pathogenesis.