Regulation of the macrolide-lincosamide-streptogramin B resistance gene ermD.

摘要: The erythromycin resistance gene ermD, which encodes an rRNA methylase protein, has unusually long leader region (354 nucleotides). Previously, a single promoter-proximal peptide coding sequence was recognized from the nucleotide sequence, and erythromycin-induced ribosome stalling in this proposed to be required for induction of translation. We characterized spontaneously occurring vitro-constructed mutations effort understand function various segments ermD region. A second identified, location insertion point that expressed constitutively suggested translation is controlled by first peptide. From Northern RNA blot analysis transcription, it appears regulation expression not transcriptional attenuation. Images