Characterization of a New erm-Related Macrolide Resistance Gene Present in Probiotic Strains of Bacillus clausii

摘要: The mechanism of resistance to macrolides, lincosamides, and streptogramins B was studied in four Bacillus clausii strains that are mixed a probiotic administered humans for prevention gastrointestinal side effects due oral antibiotic chemotherapy three reference B. clausii, DSM8716, ATCC 21536, 21537. An 846-bp gene called erm(34), which is related the erm genes conferring these antibiotics by ribosomal methylation, cloned from total DNA DSM8716 into Escherichia coli. deduced amino acid sequence presented 61% identity with Erm(D) licheniformis, halodurans, anthracis. Pulsed-field gel electrophoresis digested I-CeuI, followed hybridization an erm(34)-specific probe, indicated chromosomal location all strains. Repeated attempts transfer macrolides conjugation Enterococcus faecalis JH2-2, E. faecium HM1070, subtilis UCN19 were unsuccessful.