Deletion of Genes From the Mouse Genome Using Cre/ loxP Technology

摘要: The steps required to delete genes from the mouse genome are illustrated by showing how a cluster of three flavin-containing monooxygenase (Fmo) (Fmo1, Fmo2, and Fmo4) were deleted chromosome 1. Such large deletions accomplished using loxP/Cre recombinase technology. Genomic clones corresponding be first isolated, then appropriate genomic fragments cloned into vectors containing loxP site. This produces targeting vectors, which electroporated embryonic stem (ES) cells allow homologous recombination event take place between fragment, present within vector, sequences in ES cell genome. Screening for recombinants sites have been inserted on either side gene is described. Recombination Cre produce lines carrying deletion 1 also