A new Sephadex-based method for removing microbicidal and cytotoxic residues when testing antiseptics against viruses: Experiments with a human coronavirus as a model.

摘要: The relative lack of efficient methods for evaluating antiseptic antiviral activity, together with weaknesses in the existing European Standard (i.e. NF EN 14476+A1), underlines need to seek a new method which could allow more precise evaluation activity chemical agents. This protocol is based on an original gel-based filtration method, using "in-house" G-25 and G-10 Sephadex columns. allows neutralization both cytotoxicity large range molecules, according their molecular size, only 1min. viral model used was human coronavirus (HCoV) 229E chosen (i) its increasing medical interest, (ii) potential resistance (iii) representing enveloped viruses mentioned Standard. First, validated it demonstrated that fully operational potentiality useful screen potentially molecules. Second, chlorhexidine (CHX) hexamidine (HXM) were assessed anti-HCoV activities. It clearly HXM had no HCoV CHX showed moderate but insufficient be antiseptic.