Fibroblast growth factor 9 (FGF9) regulation of cyclin D1 and cyclin-dependent kinase-4 in ovarian granulosa and theca cells of cattle.

摘要: Abstract To determine the mechanism by which fibroblast growth factor 9 (FGF9) alters granulosa (GC) and theca (TC) cell proliferation, cycle proteins that regulate progression through G1 phase of cycle, cyclin D1 (CCND1) cyclin-dependent kinase-4 (CDK4; CCND1's catalytic partner), were evaluated. Ovaries obtained from a local abattoir, GC harvested small (1–5 mm) large (8–22 mm) follicles, TC follicles. plated in medium containing 10% fetal calf serum followed various treatments serum-free medium. Treatment with 30 ng/mL either FGF9 or IGF1 significantly increased numbers when combined, synergized to further increase threefold. Abundance CCND1 CDK4 mRNA quantified via real-time PCR. Alone combination IGF1, expression both TC. Western blotting revealed protein levels after 6 h 12 h treatment, but was not affected. A mitogen-activated kinase (MAPK)/extracellular signal-regulated (ERK) pathway inhibitor, U0126, reduced FGF9-induced basal levels. For first time we show is bovine GC, likely uses MAPK induce production