作者: Bruna Martins Dellagnezze , Gabriel Vasconcelos de Sousa , Laercio Lopes Martins , Daniela Ferreira Domingos , Elmer E.G. Limache
DOI: 10.1016/J.MARPOLBUL.2014.10.003
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摘要: Bacterial strains and metagenomic clones, both obtained from petroleum reservoirs, were evaluated for degradation abilities either individually or in pools using seawater microcosms 21 days. Gas Chromatography-Flame Ionization Detector (GC-FID) Chromatography-Mass Spectrometry (GC-MS) analyses carried out to evaluate crude oil degradation. The results showed that clones 1A 2B able biodegrade n-alkanes (C14 C33) isoprenoids (phytane pristane), with rates ranging 31% 47%, respectively. bacteria Dietzia maris CBMAI 705 Micrococcus sp. 636 higher reaching 99% after clone pool biodegraded these compounds at 11% 45%. Regarding aromatic compound biodegradation, 10A up 94% of phenanthrene methylphenanthrenes (3-MP, 2-MP, 9-MP 1-MP) 55% 70% days, while the 63% phenanthrene, respectively, 23% In this work, isolated as well capable degrading several compounds, revealing an innovative strategy a great potential further biotechnological bioremediation applications.