Capillary Isoelectric Focusing

摘要: Capillary isoelectric focusing (CIEF) is a widespread technique for the analysis of peptides and proteins in biological samples. CIEF used to separate mixtures compounds on basis differences their point. Aspects sample preparation, capillary selection, zone mobilization procedures as well various detection modes have been described discussed. Moreover CIEF, coupled types techniques (MALDI or LIF), has increasingly applied variety different high-molecular compounds. considered highly specific analytical method which may be routinely separation rare hemoglobin variants. In addition, application proteomic field discussed examples analyses glycoproteins immunoglobins due meaning clinical diagnostic.