H(+) V-ATPase-energized transporters in brush border membrane vesicles from whole larvae of Aedes aegypti.

作者: William R. Harvey , Bernard A. Okech , Paul J. Linser , James J. Becnel , Gregory A. Ahearn

DOI: 10.1016/J.JINSPHYS.2010.04.017

关键词:

摘要: Abstract Brush border membrane vesicles (BBMVs) from Whole larvae of Aedes aegypti (AeBBMVWs) contain an H+ V-ATPase (V), a Na+/H+ antiporter, NHA1 (A) and Na+-coupled, nutrient amino acid transporter, NAT8 (N), VAN for short. All subunits are present in the Ae. genome vesicles. AgNAT8 was cloned Anopheles gambiae, localized BBMs characterized Xenopus laevis oocytes. AgNHA1 but characterization oocytes compromised by endogenous cation conductance. AeBBMVWs complement characterizing proteins, can be energized voltage their natural lipid environment. BBMVs caterpillars were used radio-labeled solute uptake experiments ∼10,000 mosquito needed to equal 10 caterpillars. By contrast, functional prepared 10,000 whole 4 h. Na+-coupled 3Hphenylalanine mediated AeNAT8 AeBBMVs compared Phe-induced inward Na+ currents Western blots light micrographs samples taken during AeBBMVW isolation labeled with antibodies against all components. The use study coupling between electrogenic V-ATPases electrophoretic transporters is discussed.

参考文章(71)
E. A. Meleshkevitch, Molecular characterization of the first aromatic nutrient transporter from the sodium neurotransmitter symporter family The Journal of Experimental Biology. ,vol. 209, pp. 3183- 3198 ,(2006) , 10.1242/JEB.02374
Scott M. Lewis, David D. Thomas, Resolved conformational states of spin-labeled calcium-ATPase during the enzymic cycle Biochemistry. ,vol. 31, pp. 7381- 7389 ,(1992) , 10.1021/BI00147A024
Etana Padan, Eitan Bibi, Masahiro Ito, Terry A. Krulwich, Alkaline pH homeostasis in bacteria: New insights Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 1717, pp. 67- 88 ,(2005) , 10.1016/J.BBAMEM.2005.09.010
Peter M. Piermarini, Dirk Weihrauch, Heiko Meyer, Markus Huss, Klaus W. Beyenbach, NHE8 is an intracellular cation/H+ exchanger in renal tubules of the yellow fever mosquito Aedes aegypti. American Journal of Physiology-renal Physiology. ,vol. 296, ,(2009) , 10.1152/AJPRENAL.90564.2008
W. R. Harvey, Voltage coupling of primary H+ V-ATPases to secondary Na+- or K+-dependent transporters. The Journal of Experimental Biology. ,vol. 212, pp. 1620- 1629 ,(2009) , 10.1242/JEB.031534
Wanyoike Kang'ethe, Karlygash G. Aimanova, Ashok K. Pullikuth, Sarjeet S. Gill, NHE8 mediates amiloride-sensitive Na+/H+ exchange across mosquito Malpighian tubules and catalyzes Na+ and K+ transport in reconstituted proteoliposomes American Journal of Physiology-renal Physiology. ,vol. 292, ,(2007) , 10.1152/AJPRENAL.00487.2005
Christopher L. Brett, Mark Donowitz, Rajini Rao, Evolutionary origins of eukaryotic sodium/proton exchangers. American Journal of Physiology-cell Physiology. ,vol. 288, ,(2005) , 10.1152/AJPCELL.00360.2004