作者: William R. Harvey , Bernard A. Okech , Paul J. Linser , James J. Becnel , Gregory A. Ahearn
DOI: 10.1016/J.JINSPHYS.2010.04.017
关键词:
摘要: Abstract Brush border membrane vesicles (BBMVs) from Whole larvae of Aedes aegypti (AeBBMVWs) contain an H+ V-ATPase (V), a Na+/H+ antiporter, NHA1 (A) and Na+-coupled, nutrient amino acid transporter, NAT8 (N), VAN for short. All subunits are present in the Ae. genome vesicles. AgNAT8 was cloned Anopheles gambiae, localized BBMs characterized Xenopus laevis oocytes. AgNHA1 but characterization oocytes compromised by endogenous cation conductance. AeBBMVWs complement characterizing proteins, can be energized voltage their natural lipid environment. BBMVs caterpillars were used radio-labeled solute uptake experiments ∼10,000 mosquito needed to equal 10 caterpillars. By contrast, functional prepared 10,000 whole 4 h. Na+-coupled 3Hphenylalanine mediated AeNAT8 AeBBMVs compared Phe-induced inward Na+ currents Western blots light micrographs samples taken during AeBBMVW isolation labeled with antibodies against all components. The use study coupling between electrogenic V-ATPases electrophoretic transporters is discussed.