Review: properties and assembly mechanisms of ND10, PML bodies, or PODs.

摘要: Nuclear domain 10 (ND10), also referred to as PML bodies or PODs, are discrete interchromosomal accumulations of several proteins including and Sp100. We describe here developments in the visualization ND10 mechanism assembly made possible by identification that essential for this process using cell lines lack individual ND10-associated proteins. is critical proper localization all other under physiological conditions. Introducing into a -/- line transient expression fusion with PML-producing cells recruited de novo formed ND10, attesting its nature formation. This recruitment includes Daxx, protein can bind highly enriched condensed chromatin absence PML. The segregation Daxx from increased accumulation Sentrin/SUMO-1 modified suggests presence variable equilibrium between these two nuclear sites. These findings identify basic requirements formation suggest dynamic domains controlled SUMO-1 modification state Additional adapter suggested exist behavior Sp100, Sp100 will provide basis their identification. Further information about balance actual site functional activity establish whether function homeostatic regulators only storage excess destined turnover.