[44] Elastase assays

摘要: Abstract Two methods of P. aeruginosa elastase purification are described: Method 1 involves concentration sample supernatants, followed by DEAE-Sepharose liquid chromatography, whereas 2 initial fractionations molecular sieving and hydrophobic interaction high-performance chromatography. The choice depends on the available equipment supplies. assaying activity described useful for a variety applications. elastin-nutrient agar plate method is qualitative assay to determine presence produced given culture or colony. Use quantitative elastin-Congo red appropriate determining activities mid-to-high elastase-producing cultures. For more sensitive determinations activity, use fluorogenic substrate advisable.