A simple procedure for the detection of plant extracellular proteolytic enzymes
作者： J. Stano , P. Kovacs , I. Safarik , D. Kakoniova , M. Safarikova
关键词: Extracellular 、 Gelatin 、 Agar plate 、 Biochemistry 、 Biology 、 Callus 、 Hydrolysis 、 Peptide 、 Plant cell 、 Proteolytic enzymes
摘要: A simple procedure for the detection of extracellular plant proteolytic enzymes using insoluble dye stained gelatin substrates incorporated into an appropriate culture medium is described. Extracellular proteinases produced by tested cells (callus and cell suspension) hydrolyzed dyed peptide fragments were released. Dyed zones around under proteinase-producing callus cultures formed on agar medium. Similarly, coloration media suspensions was observed.
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