Spectrophotometric determination of proteolytic activity in coloured solutions.
作者： Ivo Sˇafarˇík
DOI: 10.1016/0165-022X(89)90026-2
关键词:
摘要: A simple procedure for spectrophotometric determination of proteolytic activity in coloured solutions is described. Gelatin cross-linked with glutaric dialdehyde or formaldehyde the presence black drawing ink used as an insoluble chromolytic substrate. The absorbances reaction mixture filtrates are read near infra-red region (at 800-900 nm) where majority substances does not absorb; on contrary released from substrate during proteolysis absorbs strongly even at these wavelengths.
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sci-hub.se 参考文章(3)
Gebhard F.B. Schumacher, Wolf-Bernhard Schill, Radial diffusion in gel for micro determination of enzymes. II. Plasminogen activator, elastase, and nonspecific proteases. Analytical Biochemistry. ,vol. 48, pp. 9- 26 ,(1972) , 10.1016/0003-2697(72)90165-0
M.T. Gisslow, B.C. McBride, A rapid sensitive collagenase assay. Analytical Biochemistry. ,vol. 68, pp. 70- 78 ,(1975) , 10.1016/0003-2697(75)90680-6
Ivo Šafařik, A low-cost insoluble chromolytic substrate for the determination of proteolytic activity Biochemical Education. ,vol. 17, pp. 154- 155 ,(1989) , 10.1016/0307-4412(89)90103-9