An insoluble chromolytic substrate for the determination of proteolytic activity

作者： Ivo Šafařik

DOI: 10.1016/0165-022X(88)90003-6

关键词: Trypsin 、 Polyacrylamide gel electrophoresis 、 Casein 、 Chromatography 、 Chemistry

摘要: Abstract A new insoluble chromolytic substrate for the determination of proteolytic activity was prepared by immobilization dyed casein into structure polyacrylamide gel. The could detect approximately 0.1 μg trypsin per ml. spontaneous leakage molecules in water solutions negligible.

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