Demonstration of Activity of α-Galactosidase Secreted by Cucumis sativus L. Cells

摘要: A simple, rapid and reproducible procedure for the identification of extracellular cucumber (Cucumis sativus L.) α-galactosidase is described using callus cultures seedlings from tested plant, hairy roots 2-day-old germinating on agar plates as well cell suspension derived cultures. For determination intracellular activities α-galactosidase, 6-bromo-2-naphthyl--α--D-galactopyranoside /? -nitrophenyl- «-/ ->-galactopyranoside, respectively, were used synthetic substrates. The activity was identified by evaluating dye-zones in medium. enzyme seedling roots, cultivated supplemented with 6-bromo-2-naphthyl-α-D-galactopyranoside, hydrolyzed this substrate releasing 6-bromo-2-naphthol. By simultaneous coupling hexazonium p-rosaniline corresponding azodye formed. Thus, detected presence reddish-brown zones around plant material. parallel determined results show a 44.6% 55.4% distribution activity. plate method enables rapid, simple specific detection producers α-galactosidase.