Fabrication and Modeling of an Ultrasensitive Label Free Impedimetric Immunosensor for Aflatoxin B1 based on Protein A Self-assembly Modified Gold 3D Nanotube Electrode ensembles

摘要: Abstract A novel label-free impedimetric immunosensor for the quantitative detection of aflatoxin B1 (AFB1) was developed based on orientated staphylococcus protein (SPA) modified gold three dimensional nanotube ensembles (3DTNEEs) which consisted nanotubes fabricated by combining technologies template synthesis and controlled chemical etching track-etched polycarbonate membrane. The structure morphology 3DTNEEs were evaluated scanning electron microscopy. diffusion characteristic local current distribution simulated via COMSOL Multiphysics 4.4 simulative software. SPA self-assembly (SPA/3DTNEEs) assessed X-ray photoelectron spectroscopy. Electrochemical impedance spectroscopy employed to characterize fabrication process optimize working conditions. model molecular recognition interface constructed analyzed. limit AFB1 1.0 × 10−12 g mL−1 (S/N = 3) with a linear concentration range 4.0 × 10−12 6.0 × 10−9 g mL−1. recovery test in corn samples demonstrated one potential application immunosensor.