Expression Profiles of psbA, ALS, EPSPS, and Other Chloroplastic Genes in Response to PSII-, ALS-, and EPSPS-Inhibitor Treatments in Kochia scoparia

摘要: Kochia (Kochia scoparia L. Schrad.), also known as tumbleweed, is an economically important annual C4 broadleaf weed found throughout the US Great Plains. Several herbicides with different modes of action are used in management of kochia. The effect commonly on expression their target site(s) and photosynthetic/chloroplastic genes poorly understood in weed species, including kochia. objective this research was to characterize profiles of herbicide target-site genes, KspsbA, KsALS, KsEPSPS upon treatment PSII- (e.g. atrazine), ALS- (e.g. chlorsulfuron), EPSPS- glyphosate)-inhibitors, respectively, kochia. Furthermore, involved photosynthesis KsRubisco, KsCAB, KsPPDK) also determined response these herbicide treatments. KspsbA strongly upregulated (>200-fold) 24 h after atrazine treatment. Transcript levels KsALS or were 7 and 3-fold higher chlorsulfuron glyphosate treatment, respectively. a Calvin cycle gene important for CO2 fixation, 2.6-fold 8 after glyphosate treatments, whereas it downregulated h after atrazine treatment. transcript KsPPDK remained unchanged but increased 1.8-fold decreased 2-fold at atrazine treatments, KsCAB results show that treatments not only affect respective expression, influence the critical photosynthetic pathway.