Estudo da influência do solvente, carboidrato e ácido graxo na síntese enzimática de ésteres de açúcares

摘要: The aim of this work was to gain knowledge enzymatic processes for the synthesis fatty acid esters sugar, with objective develop an process preparation non-toxic biodegradable surface-active agents derived entirely from renewable resources. A wide range data were collected reaction conditions involving different sugars (glucose, fructose and sucrose), acids (oleic, palmitic, lauric), solvents (hexane, heptane t-butanol) sources lipases in both free immobilized forms. As a solvent t-butanol provided best create catalytic liquid phase which occurs. Sugars preferentially esterified following order: > glucose sucrose, depending on enzyme preparation. For no influence found concerning de acyl donor similar rates achieved all tested acids. Ester maximized substrates containing fructose, lauric or oleic acids, lipase porcine pancreas polysiloxane-polyvinyl alcohol particles. Under such molar conversions higher than 50%.