Temperature stress differentially modulates transcription in meiotic anthers of heat-tolerant and heat-sensitive tomato plants.

摘要: Fluctuations in temperature occur naturally during plant growth and reproduction. However, the hot summers this variation may become stressful damaging for molecular mechanisms involved proper cell growth, impairing thus development particularly fruit-set many crop plants. Tolerance to such a stress can be achieved by constitutive gene expression or rapid changes expression, which ultimately leads protection against thermal damage. We have used cDNA-AFLP microarray analyses compare early response of tomato meiotic anther transcriptome moderate heat conditions (32°C) heat-tolerant heat-sensitive genotype. In light expected global increases, elucidating protective identifying candidate tolerance genes improve breeding strategies stress. The analysis shows that 30 h (MHS) alter approximately 1% studied transcript-derived fragments major effect is down-regulation after first 2 subsequently applied elucidate responses genotype, also about having significant tolerant genotype not only reacts with transcriptomic but exhibits constitutively higher levels thermotolerance. contrast transcriptional under Moreover, different profile compared indicating genetic differences adaptation increased temperatures. majority represented up-regulation, while there general trend down-regulate upon MHS. putative functions associated identified indicate involvement shock, metabolism, antioxidant pathways. Based on observed MHS literature sources, we number transcripts heat-tolerance.