Cytokine Panels and Pediatric Acute Respiratory Distress Syndrome: A Translational Investigation.

摘要: Objectives To identify and compare serum lower respiratory tract fluid biomarkers of lung injury using well-characterized mouse models injury. explore the relationship between these preclinical clinical outcomes in a discovery cohort pediatric patients with acute failure from pneumonia. Design Prospective, observational study. Setting A basic science laboratory PICU tertiary-care children's hospital. Patients intubated for suspected infection. Interventions Prospective enrollment collection samples. Measurements main results C57BL6/J mice were intranasally inoculated escalating doses influenza virus or toll-like receptor agonists to simulate varying degrees Serum bronchoalveolar lavage measured presence cytokines commercially available multiplex cytokine assays. Elevated levels C-C motif chemokine ligand 7 at peak inflammation both correlated lethality, ratio 7:C-C 22 providing best prediction models. These examined plasma The primary outcome measure was ventilator-free days, secondary distress syndrome severity mortality. Elevation ratios demonstrated significant negative correlation days (r = -0.805; p Conclusions This study provides evidence that immune profiling via analysis is feasible may provide insight into outcomes. Further validation markers, including this limited study, larger necessary.