Advantages and pitfalls of radioimmune and enzyme linked immunosorbent assays of insulin antibodies.

作者: F. Sodoyez-Goffaux , M. Koch , N. Dozio , D. Brandenburg , J-Cl. Sodoyez

DOI: 10.1007/BF00278754

关键词:

摘要: Human sera were tested for insulin antibodies by fluid and solid phase assays. Radioimmune titres determined with 125-I Tyr A14 not correlated those obtained using coated microplates enzyme linked immunodetection (n=60). Several reasons this lack of correlation found. Iodine substitution on the residue may significantly alter avidity some their ligand; hence, disclosing a heretofore unsuspected pitfall antibody determination radio-immunoassay. Specificity bovine was easily demonstrable in comparing binding monoiodinated bovine, porcine human insulin. By contrast, assay, or almost equal, regardless serum specificity This assay is due to denaturation unavailability specific epitope because: could be demonstrated competitive after preincubation different species; and, coating crosslinked dimers oligomers instead monomers did unmask specificity. It concluded that radioimmune methods are best suited study but biased presence radioiodine label whereas detects low great efficiency less appropriate

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