Proteins in renal stones and urine of stone formers.

摘要: Knowledge of the essential characteristics macromolecules constituting organic matrix nidus urinary stones is required to understand mechanism urolithogenesis. The aim this study was isolate and characterise those stone proteins. Using an extraction buffer containing SDS β-mercaptoethanol, we were able overcome known problems protein isolation from matrix. These proteins characterised by a strong tendency aggregate under reducing denaturing conditions. On SDS-PAGE, their molecular weights range ≤12 66 kDa. Antisera raised against showed cross-reactivity between isolated different irrespective origin or mineral composition. Moreover, formers also cross-reacted with these whereas there no reaction non-stone formers. Western blotting confirmed findings. Given above summarised properties, it can be safely concluded that are prevalent in urines formers, they selectively incorporated into renal all aetiologies, most likely have role and, therefore, early formation.