Second prize: Comprehensive proteomic analysis of human calcium oxalate monohydrate kidney stone matrix.
作者： Benjamin K. Canales , Lorraine Anderson , Leeann Higgins , Joel Slaton , Ken P. Roberts
关键词: CALCIUM OXALATE MONOHYDRATE 、 Oxalate 、 Medicine 、 Mass spectrometry 、 Kidney stones 、 Proteomics 、 Chromatography 、 Matrix (chemical analysis) 、 Peptide 、 Tandem mass spectrometry
摘要: Background and Purpose: Previous efforts to identify the protein content of stone matrix have been limited by lack technology necessary analyze highly insoluble protein-crystalline complex. Our study objective is characterize calcium oxalate monohydrate (COM) stones using a comprehensive proteomics approach. Materials Methods: Seven pure COM were powdered, proteins extracted four different buffer solutions. Detergent cleanup spin columns or concentrators used remove detergent exchange buffers before trypsin digestion. Tryptic peptides analyzed with reversed-phase, high-performance liquid chromatography (RP-HPLC) tandem mass spectrometry (MS/MS) QSTAR Pulsar i quadrapole time flight spectrometer. Tandem spectra searched against National Center for Biotechnology Information human nonredundant database ProteinPilot™ 1.0 software (Applied Biosystems, Inc.) hits; peptide MS/MS we...
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