Oxalate toxicity in LLC-PK1 cells: Role of free radicals

摘要: Oxalate toxicity in LLC-PK 1 cells: Role of free radicals. Oxalate, the most common constituent kidney stones, is an end product metabolism that excreted by kidney. During excretion, oxalate transported a variety transport systems and accumulates renal tubular cells. This process has been considered benign; however, recent studies on cells suggested high concentrations are toxic, inducing morphological alterations, increases membrane permeability to vital dyes loss from monolayer cultures. The present examined basis for toxicity, focusing possibility exposure might increase production/availability radicals Free radical production was monitored two ways, monitoring reduction nitroblue tetrazolium blue reaction following conversion dihydrorhodamine 123 (DHR) its fluorescent derivative, rhodamine 123. Such demonstrated induces concentration-dependent dye sensitive scavengers. Specifically, addition catalase or superoxide dismutase blocked oxalate-induced changes fluorescence/absorbance. Addition these scavengers also prevented integrity Thus it seems likely responsible toxicity. levels induced (350 µ m) only slightly higher than would be expected occur cortex. These considerations suggest hyperoxaluria may contribute progression injury several forms disease.