Genetic evidence for two distinct transactivation functions of the herpes simplex virus alpha protein ICP27.

摘要: Abstract Infected-cell protein 27 (ICP27) is a herpes simplex virus type 1 alpha, or immediate-early, involved in the regulation of viral gene expression. To better understand function(s) ICP27 infected cells, we have isolated and characterized recombinants containing defined alterations gene. The mutant d27-1 contains 1.6-kilobase deletion which removes promoter most coding sequences, while n59R, n263R, n406R, n504R are mutants nonsense mutations encode molecules truncated at their carboxyl termini. All five were defective for lytic replication Vero cells. Analysis phenotypes suggests that has following regulatory effects during infection: (i) stimulation expression gamma-1 genes, (ii) induction gamma-2 (iii) down alpha beta genes late infection, (iv) DNA replication. Cells with expressed wild-type levels proteins but appeared to be unable efficiently express mRNAs proteins. This result mediates two distinct transactivation functions, one stimulates second required induction. n406R suggested polypeptide can interfere many genes. Our results demonstrate variety positive negative on infection.