Deletion mutants in the gene encoding the herpes simplex virus type 1 immediate-early protein ICP0 exhibit impaired growth in cell culture.

摘要: We report the construction and characterization of deletion mutants in herpes simplex virus type 1 gene encoding immediate-early protein ICP0. In event that ICP0 proved to play an essential role replication, ICP0-transformed Vero cells were generated serve as permissive hosts for such mutants. Two mutants, dlX0.7 dlX3.1, isolated these by a marker rescue-transfer procedure involving rescue ICP4 mutant simultaneous insertion linked gene. Mutant contained 700-base-pair both copies The lay entirely within transcript specified induced synthesis ICP0-specific mRNA was approximately 0.7 kilobases smaller than corresponding wild-type virus. 3.1-kilobase dlX3.1 removed majority transcriptional-regulatory signals coding sequences, retaining only sequences at 3' end As expected, no detected dlX3.1-infected Nero (G418-resistant cells). Both grew all tested, although their burst sizes 10- 100-fold lower Although plaque equally small on cells, plating efficiency 15- 50-fold greater cells. exhibited modest interference with growth mixed infections, effect abolished UV irradiation implying required viral expression. Polypeptide profiles qualitatively similar Quantitatively, slight reductions levels certain late polypeptides observed, phenomenon also borne out analysis glycoproteins. significant, reduced, DNA relative Taken together, results demonstrate is not productive infection cell culture but this plays significant growth, indicated impaired abilities replicate.