Development of Herpes Simplex Virus as a Gene Transfer Vector for the Central Nervous System

摘要: Herpes simplex virus (HSV) is a large neurotropic containing 152 kb of linear dsDNA encoding at least 70 gene products. In natural human infections, the replicates primarily in skin or mucosal epithelial cells prior to entering regional axon terminals and retrograde transport capsid sensory ganglia, where it may either replicate establish latency (Roizman Sears 1990; Fig. 1). Latent can be reactivated by variety stimuli followed anterograde productive secondary infection near primary site. animals models, peripheral ganglia achieved through inoculation (Cook Stevens 1973), cornea (Sciler Schwab 1984) olfactory bulb after intranasal (Dobson et al. 1989). Focal specific brain regions stereotactic injection small volumes into defined (McFarland 1986; Bak 1977).