Glucocorticoid-induced insulin resistance in vitro: evidence for both receptor and postreceptor defects.

摘要: Glucocorticoid excess in vivo has been shown to cause decreases insulin sensitivity and receptor binding target tissues. It not previously possible produce all of these changes vitro because limitations the isolated rat adipocyte as an experimental model. We have studied effects glucocorticoid hormones on action differentiated mouse 3T3-L1 fatty fibroblasts, attempt develop a complete model resistance associated with excess. Exposure cells dexamethasone, synthetic glucocorticoid, resulted decreased its stimulation 2-deoxyglucose uptake. Although some effect dexamethasone parameters was observed after 24 h, more than 3 days were required for maximal inhibition both biological response. The magnitude concentration dependent over range 0.1 –100 nM dexamethasone. Ex...