Microsatellite instability and frameshift mutations in genes involved in cell cycle progression or apoptosis in ovarian cancer.

摘要: The loss of mismatch repair enzymes increases the mutation rate in microsatellites and coding regions genome appears to be involved drug resistance. replication error (RER+) phenotype, associated with microsatellite instability, has been widely described for both familial sporadic colon cancers gastric endometrial tumors. For ovarian cancer, incidence RER+ cases among tumors is still uncertain. We analyzed epithelial carcinoma cell lines instability mutations different genes, including recently discovered human CHK-1 gene, which an important role controlling cycle progression whose region contains a poly(A)9 tract. Microsatellite frameshift BAX, TGFbetaRII, IGFIIR, E2F-4, ICE, genes were cancer samples by polymerase chain reaction (PCR). Approximately 26% patients showed two or more loci. BAT-26 locus no alteration primary detected BAX one tumor sample TGFbetaRII line. Our findings confirm presence phenotype cancer. low previously reported altered suggests that other not yet identified might could play response treatment