作者: Navin Jain , Arpit Bhargava , Mohit Rathi , R. Venkataramana Dilip , Jitendra Panwar
DOI: 10.1371/JOURNAL.PONE.0134337
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摘要: The present study demonstrates an economical and environmental affable approach for the synthesis of “protein-capped” silver nanoparticles in aqueous solvent system. A variety standard techniques viz. UV-visible spectroscopy, transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) X-ray diffraction (XRD) measurements were employed to characterize shape, size composition nanoparticles. synthesized found be homogenous, spherical, mono-dispersed covered with multi-layered protein shell. In order prepare bare nanoparticles, shell was removed from biogenic as confirmed by FTIR photoluminescence analysis. Subsequently, antibacterial efficacy protein-capped compared bacterial growth rate minimum inhibitory concentration assay. results revealed that more effective varying potential against tested Gram positive negative species. Mechanistic studies based on ROS generation membrane damage suggested demonstrate distinct mode action. These findings strengthened TEM imaging along ion release using inductively coupled plasma atomic emission (ICP-AES). conclusion, our illustrate presence can decrease their bactericidal effects. open new avenues surface modifications modulate enhance functional properties.