Removal of Protein Capping Enhances the Antibacterial Efficiency of Biosynthesized Silver Nanoparticles.

作者: Navin Jain , Arpit Bhargava , Mohit Rathi , R. Venkataramana Dilip , Jitendra Panwar

DOI: 10.1371/JOURNAL.PONE.0134337

关键词:

摘要: The present study demonstrates an economical and environmental affable approach for the synthesis of “protein-capped” silver nanoparticles in aqueous solvent system. A variety standard techniques viz. UV-visible spectroscopy, transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) X-ray diffraction (XRD) measurements were employed to characterize shape, size composition nanoparticles. synthesized found be homogenous, spherical, mono-dispersed covered with multi-layered protein shell. In order prepare bare nanoparticles, shell was removed from biogenic as confirmed by FTIR photoluminescence analysis. Subsequently, antibacterial efficacy protein-capped compared bacterial growth rate minimum inhibitory concentration assay. results revealed that more effective varying potential against tested Gram positive negative species. Mechanistic studies based on ROS generation membrane damage suggested demonstrate distinct mode action. These findings strengthened TEM imaging along ion release using inductively coupled plasma atomic emission (ICP-AES). conclusion, our illustrate presence can decrease their bactericidal effects. open new avenues surface modifications modulate enhance functional properties.

参考文章(63)
FrankW. Fales, THE ASSIMILATION AND DEGRADATION OF CARBOHYDRATES BY YEAST CELLS Journal of Biological Chemistry. ,vol. 193, pp. 113- 124 ,(1951) , 10.1016/S0021-9258(19)52433-4
Hong Wang, James A Joseph, Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radical Biology and Medicine. ,vol. 27, pp. 612- 616 ,(1999) , 10.1016/S0891-5849(99)00107-0
John A. Buege, Steven D. Aust, Microsomal lipid peroxidation. Methods in Enzymology. ,vol. 52, pp. 302- 310 ,(1978) , 10.1016/S0076-6879(78)52032-6
David W Brett, A DISCUSSION OF SILVER AS AN ANTIMICROBIAL AGENT: ALLEVIATING THE CONFUSION Ostomy Wound Management. ,vol. 52, pp. 34- 41 ,(2006)
Renata de Lima, Amedea B. Seabra, Nelson Durán, Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles Journal of Applied Toxicology. ,vol. 32, pp. 867- 879 ,(2012) , 10.1002/JAT.2780
Petr Suchomel, Libor Kvitek, Ales Panacek, Robert Prucek, Jan Hrbac, Renata Vecerova, Radek Zboril, Comparative Study of Antimicrobial Activity of AgBr and Ag Nanoparticles (NPs) PLOS ONE. ,vol. 10, pp. e0119202- ,(2015) , 10.1371/JOURNAL.PONE.0119202
Wentong Lu, Dulal Senapati, Shuguang Wang, Oleg Tovmachenko, Anant Kumar Singh, Hongtao Yu, Paresh Chandra Ray, Effect of Surface Coating on the Toxicity of Silver Nanomaterials on Human Skin Keratinocytes. Chemical Physics Letters. ,vol. 487, pp. 92- 96 ,(2010) , 10.1016/J.CPLETT.2010.01.027
Nadanathangam Vigneshwaran, Arati A. Kathe, P.V. Varadarajan, Rajan P. Nachane, R.H. Balasubramanya, Biomimetics of silver nanoparticles by white rot fungus, Phaenerochaete chrysosporium Colloids and Surfaces B: Biointerfaces. ,vol. 53, pp. 55- 59 ,(2006) , 10.1016/J.COLSURFB.2006.07.014
H. C. Neu, The Crisis in Antibiotic Resistance Science. ,vol. 257, pp. 1064- 1073 ,(1992) , 10.1126/SCIENCE.257.5073.1064
Zong-ming Xiu, Qing-bo Zhang, Hema L. Puppala, Vicki L. Colvin, Pedro J. J. Alvarez, Negligible Particle-Specific Antibacterial Activity of Silver Nanoparticles Nano Letters. ,vol. 12, pp. 4271- 4275 ,(2012) , 10.1021/NL301934W