Cloning and Expression of Genes Encoding the nupG Nucleoside Transport System in Escherichia Coli
作者： Agnete Munch-Petersen , Nina Jensen
DOI: 10.1007/978-1-4684-1215-4_9
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摘要: A DNA fragment, directing the synthesis of nupG nucleoside transport system, has been cloned on a plasmid vector as evidenced by complementation chromosomal mutations. The encoded system responded to cytR and deoR control systems, which are known act system. Plasmid-containing minicells were used for specific in vitro proteins. Two proteins, with molecular mass approximately 10,000 80,000, detected, occurrence regulation suggested that they components
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