Hydrolysis of 2-Hydroxy-4-(Methylthio)Butanoic Acid Dimer in Two Model Systems

摘要: Hydrolysis of 2-hydroxy-4-(methylthio)butanoic acid (HMB) dimer was observed in two model systems that were designed to emulate vivo conditions. A physiologically feasible rate hydrolysis this methionine precursor has not previously been demonstrated vivo. In the first model, simulated intestinal fluid (SIF) used, and second sections chick small intestine incubated vitro with compound under study. When HMB SIF, very rapid until half hydrolyzed. Subsequent negligible system. contrast, neared completion when dimer. These results indicate studies cleavage must include consideration enzymatic factors.