Processing Respiratory Specimens with C18-Carboxypropylbetaine: Development of a Sediment Resuspension Buffer That Contains Lytic Enzymes To Reduce the Contamination Rate and Lecithin To Alleviate Toxicity

摘要: The C18-carboxypropylbetaine (CB-18) procedure for processing respiratory specimens the detection of mycobacteria was shown to provide significant increases in sensitivity by smear and culture. However, also produced increased contamination, a loss liquid culture sensitivity, reduction specificity. Because these observations, toxicity CB-18 nature contamination were characterized. Preincubation 1 mM impacted viability time-dependent fashion, but magnitude species isolate dependent. Mycobacterium tuberculosis isolates most susceptible, losing 20 30% CFU within 30 min 60% after 3 h, whereas avium fortuitum unaffected CB-18. In culture, when concentration exceeded 5 μg/ml, there an impact on growth characteristics susceptible M. isolate. contrast, able grow 100 μg per ml. deleterious effects enhanced presence antibiotics, solid media not similarly affected. Supplementation resuspension buffer with 0.15% lecithin alleviated toxicity. Initial attempts modify control incorporated acids or alkalis; however, losses occurred. Studies identify contaminants led development sediment that contained lytic enzymes combat alleviate This formulation included lysozyme, zymolyase, Cytophaga Trichoderma extracts seen reduce acceptable levels (<5%).