Upstream processes in antibody production: evaluation of critical parameters.

摘要: The demand for monoclonal antibody therapeutic and diagnostic applications is rising constantly which puts up a need to bring down the cost of its production. In this context it becomes prerequisite improve efficiency existing processes used This review describes various upstream production evaluates critical parameters efforts are being made enhance process. technology has tremendously been upgraded from host cells manufacturing bioreactors type capacity. range microbial, mammalian plant with dominating scenario. Disposable promoted small scale due easy adaptation process validation flexibility, though they limited by respect Wave suspension culture have introduced recently. A novel bioreactor immobilized described permits an economical alternative hollow fiber at lab Modification cellular machinery alter their metabolic characteristics further added robustness perks cell specific productivity. including feeding strategies environmental improved validate them get reproducible results becoming trend. Online monitoring product characterization increasingly gaining importance. total advancement led increase in volumetric productivity 100-fold over last decade make more realistic option applications.