Growth kinetics of hybridoma cells: (2) The effects of varying energy source concentrations.

摘要: Commercial media used for the growth of hybridoma cells contain different glucose concentrations. We have attempted to establish optimal levels required maximum cell yields by studying energy source metabolism two murine lines in several commercial static 25 cm2 flask cultures. The and lactate quotients from were similar all media. Glucose limited DME containing lg/L. supplementation 2g/L significantly increased antibody yields. When fructose was as a substitute quotient one line found be its whereas that other reduced compared quotient.