Media replenishment: A tool for the analysis of high-cell density perfusion systems

作者: Mina D. Mahadevan , John A. Klimkowsky , Yashwant M. Deo

DOI: 10.1007/BF00758173

关键词:

摘要: A simple method to analyze media consumption and by-product build-up in high-cell-density perfusion cultures has been developed. This technique makes use of a replenishment strategy which can be used decouple the two phenomena. Also, replenished identify limitation by complex undefined components such as bovine serum.

参考文章(7)
Low K, Harbour C, Growth kinetics of hybridoma cells: (2) The effects of varying energy source concentrations. Developments in biological standardization. ,vol. 60, pp. 73- 79 ,(1985)
Michael W. Glacken, Catabolic Control of Mammalian Cell Culture Nature Biotechnology. ,vol. 6, pp. 1041- 1050 ,(1988) , 10.1038/NBT0988-1041
D. Duval, C. Demangel, K. Munier-Jolain, S. Miossec, I. Geahel, Factors controlling cell proliferation and antibody production in mouse hybridoma cells: I. Influence of the amino acid supply. Biotechnology and Bioengineering. ,vol. 38, pp. 561- 570 ,(1991) , 10.1002/BIT.260380602
Sadettin S. Ozturk, Mark R. Riley, Bernhard O. Palsson, Effects of Ammonia and Lactate on Hybridoma Growth, Metabolism, and Antibody Production Biotechnology and Bioengineering. ,vol. 39, pp. 418- 431 ,(1992) , 10.1002/BIT.260390408
Mina Dalili, David F. Ollis, Transient kinetics of hybridoma growth and monoclonal antibody production in serum-limited cultures. Biotechnology and Bioengineering. ,vol. 33, pp. 984- 990 ,(1989) , 10.1002/BIT.260330807
Katherine L. McKinney, Robert Dilwith, Georges Belfort, Manipulation of heterogeneous hybridoma cultures for overproduction of monoclonal antibodies. Biotechnology Progress. ,vol. 7, pp. 445- 454 ,(1991) , 10.1021/BP00011A010