G-protein-mediated Ca2+ sensitization of smooth muscle contraction through myosin light chain phosphorylation.
作者： T Kitazawa , B D Gaylinn , G H Denney , A P Somlyo
DOI: 10.1016/S0021-9258(18)52353-X
关键词:
摘要: Abstract The Ca2+ sensitivities of tonic (pulmonary and femoral artery) phasic (portal vein ileum) smooth muscles the effects guanosine 5'-O-(gamma-thiotriphosphate) (GTP gamma S) norepinephrine on sensitivity force development myosin light chain (MLC20) phosphorylation were determined in permeabilized preparations that retained coupled receptors endogenous calmodulin. was higher (approximately 3-fold) than muscles. nucleotide specificity sensitization was: GTP S much greater ITP CTP = UTP. Baseline (7% at pCa 8) maximal (58% 5.0) both lower portal artery (20 97%). Norepinephrine increased constant [Ca2+] (pCa 7.0-6.5). MLC20 induced by completely inhibited 5'-O-(beta-thiodiphosphate) (GDP beta S). In 5, from 58%, Ca2(+)-activated value, to 75%, 8, 7 13%. neither (97%) nor affected S, while caused an increase (16% maximum) with a borderline (from 20 27%). (up 100%) positively correlated force. major results support hypothesis G-protein Ca2(+)-sensitizing effect agonists is secondary phosphorylation.
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