Rapid Diagnosis of Porcine Epidemic Diarrhea Virus Infection by Polymerase Chain Reaction
作者： Chang-Hee KWEON , Jae-Gil LEE , Myung-Guk HAN , Yung-Bai KANG
DOI: 10.1292/JVMS.59.231
关键词:
摘要: The diagnosis of porcine epidemic diarrhea virus (PEDV) infection in the laboratory is rather fastidious because difficulties propagation. feasibility propagation vivo also limited by handling a number samples at same time. In this study, detection PEDV reverse transcription polymerase chain reaction (RT-PCR) described. RT-PCR could detect up to 10(4) TCID50/ml and did not show any cross with transmissible gastroenteritis or rotavirus. Using method, experimentally inoculated piglets was possible as early one day after inoculation. These results suggest that be applicable for rapid infection.
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