Early in vitro and in vivo transcription of T4 DNA. I. Effect of phage T4 infection on RNA production in Escherichia coli.

摘要: Abstract 1. The technique of acrylamide-gel electrophoresis has been used to investigate the nature RNA synthesized in vivo Escherichia coli immediately after phage T4 infection at 25°. Nucleic acid species coded for by bacteria were generally found be inhibited between 3 and 6 min processes depending on protein synthesis. 2. Two physically distinct classes messenger appear made their temporal separation suggests that they might correspond messages pre-early early proteins. This is borne out hybridization-competition data which show time syntheses corresponds synthesis two broad observed previously occur infection. 3. Gel both indicate larger from dependent