Null Mutation of the Nicotinamide Adenine Dinucleotide Phosphate–Oxidase Subunit p67phox Protects the Dahl-S Rat From Salt-Induced Reductions in Medullary Blood Flow and Glomerular Filtration Rate

摘要: Null mutations in the p67 phox subunit of nicotinamide adenine dinucleotide phosphate–oxidase confer protection from salt sensitivity on Dahl salt-sensitive rats. Here, we track sequential changes medullary blood flow (MBF), glomerular filtration rate (GFR), urinary protein, and mean arterial pressure SS null rats wild-type littermates during 21 days 4.0% NaCl high-salt (HS) diet. Optical fibers were implanted renal medulla MBF was measured conscious by laser Doppler flowmetry. Separate groups prepared with femoral venous catheters GFR transcutaneous assessment fluorescein isothiocyanate-sinistrin disappearance curves. Mean telemetry. In rats, HS caused a rapid reduction MBF, which significantly lower than control values day-6. Reduced associated progressive increase pressure, averaging 170±5 mm Hg day-21. A significant evident day-14 HS, after onset hypertension reduced MBF. contrast, had no effect pressor response to sodium blunted, 150±3 day-21 HS. maintained throughout study proteinuria reduced. summary, when is not functional does cause attenuated, consequently injury maintained.