Epigenetic Deregulation Across Chromosome 2q14.2 Differentiates Normal from Prostate Cancer and Provides a Regional Panel of Novel DNA Methylation Cancer Biomarkers

摘要: Background: Previously, we showed that gene suppression commonly occurs across chromosome 2q14.2 in colorectal cancer, through a process of long-range epigenetic silencing (LRES), involving combination DNA methylation and repressive histone modifications. We now investigate whether LRES also prostate cancer this 4-Mb region differential genes could provide regional panel biomarkers. Methods: used highly sensitive headloop PCR assays can detect 10 to 25 pg methylated with specificity at least 1:1,000, chromatin immunoprecipitation remodeling cohort 195 primary tumors 90 matched normal controls. Results: Prostate cells exhibit concordant deacetylation H3 Lysine 9 (H3K9Ac H3K9me2, respectively), localized hypermethylation EN1 , SCTR INHBB corresponding loss H3K27me3. were frequently (65% 53%, whereas was less methylated. Conclusions: Consistent found cancer. Concordant able differentiate from ( P < 0.0001) improved the diagnostic GSTP1 for detection by 26%. Impact: For first time show promoters potential novel biomarkers add increased sensitivity improve potential. Cancer Epidemiol Biomarkers Prev; 20(1); 148–59. ©2011 AACR .