Inflammation-associated gene transcription and expression in mouse lungs induced by low molecular weight compounds from fungi from the built environment.

摘要: Few metabolites from fungi found indoors have been tested for inflammatory mediators endpoints in primary cultures of alveolar macrophages or vivo. In this study, mice were intratracheally instilled with a single dose comprising 4x10(-5)moletoxin/kg lung wt either atranone C, brevianamide, cladosporin, mycophenolic acid, neoechinulin A & B, sterigmatocystin TMC-120A. These toxins are common on damp building materials. The used was comparable to the estimated doses possible human exposure. Hematoxylin and eosin (HE >or =1.5-fold < =-1.5-fold change) different treatment animal groups. Expression transcriptionally regulated genes confirmed using immunohistochemistry that demonstrated MIP-2 Tnf-alpha staining respiratory bronchiolar epithelia, type II cells. transcriptional regulation these groups suggests they may serve central roles immunomodulation toxin-induced pro-inflammatory responses. Hierarchical cluster analysis revealed significant patterns gene transcription linking response at equimolar three groups: (1) acid (2) sterigmatocystin, (3) C TMC-120. results further confirm nature metabolites/toxins such can contribute development non-allergenic health effects.