Problems and pitfalls in animal experiments designed to establish dietary requirements for essential nutrients.

摘要: Problems are encountered in nutrition research designed to establish nutrient requirements. A requirement must be defined terms of a specific criterion response for animals given age, weight, sex and body composition. The experimental diet employed carefully protein source level, energy level multitude biological availability factors, both negative (e.g., phytate fiber) positive anabolic bioactivity). Also precursor materials may contribute bioactivity the diet. Having considered these data obtained subjected appropriate statistical methods that will allow objective rather than subjective estimation maxima or minima being sought (i.e., "requirement").