Dopamine- and Tyrosine Hydroxylase-Immunoreactive Neurons in the Brain of the American Cockroach, Periplaneta americana.

摘要: The catecholamine dopamine plays several vital roles in the central nervous system of many species, but its neural mechanisms remain elusive. Detailed neuroanatomical characterization neurons is a prerequisite for elucidating dopamine’s actions brain. In present study, we investigated distribution dopaminergic brain American cockroach, Periplaneta americana, using two antisera: 1) an antiserum against dopamine, and 2) tyrosine hydroxylase (TH, enzyme required synthesis), identified about 250 putatively neurons. patterns dopamine- TH-immunoreactive were strikingly similar, suggesting that both antisera recognize same sets “dopaminergic” TH antibodies intensively or moderately immunolabeled prominent neuropils, e.g. mushroom body (memory center), antennal lobe (first-order olfactory center) complex (motor coordination center). All subdivisions exhibit immunoreactivity. Comparison with those filled by dye injection revealed group cell bodies near calyx projects into distal region vertical lobe, which plausible site memory formation insects. ordinary glomeruli as well macroglomeruli It noteworthy labeled tiny granular structures inside whereas processes marginal regions glomeruli, different origin. complex, all excluding part noduli protocerebral bridge These anatomical findings will accelerate our understanding systems, specifically circuits underlying aversive arousal,