De Novo Assembled Transcriptome Analysis and SSR Marker Development of a Mixture of Six Tissues from Lilium Oriental Hybrid ‘Sorbonne’

摘要: Lily is universally known for its large, showy, fragrant flower, and Sorbonne one of the best-selling Oriental hybrid lily cultivars. To accelerate breeding more robust, attractive, disease-resistant cultivars, it essential to introduce molecular marker-assisted breeding. However, enormous genome size, around 36 Gb, an obstacle genomic information mining. We sequenced assembled a mixture six tissues Lilium ‘Sorbonne’ using Illumina HiSeq2000. A set 49,991 Unigenes with average length 673 bp was produced, 36,093 sequences were annotated against public protein databases. The number transcripts GO annotation 26,212, 55 subcategories. Virus detected, even though in very small amounts. From Unigenes, 1,853 SSRs identified. most common repeat motifs trinucleotide dinucleotide GA/CT GGC/CCG repeats. Fifty-seven EST-SSR markers validated 32 genotypes mean PIC value 0.55. 57-primer-based dendrogram accordance genetic background derived from complex practices previous studies. Thirty out 57 SSR-derived function-known genes. Subsequently, comprehensive assembly generated, merging current transcriptome two transcriptomes available on database. distribution gene ontology three sets mismatch/gap uncovering before after analyzed. Our data are extensive complement database, which will benefit research candidate