Low-intensity pulsed ultrasound stimulates cell proliferation, proteoglycan synthesis and expression of growth factor-related genes in human nucleus pulposus cell line.

摘要: Low-intensity pulsed ultrasound (LIPUS) stimulation has been shown to effect differentiation and activation of human chondrocytes. A study involving rabbit disc cells with LIPUS revealed upregulation cell proliferation proteoglycan (PG) synthesis. However, the on nucleus pulposus not investigated. In present study, therefore, we investigated whether a line (HNPSV-1) exerted positive cellular activity. HNPSV-1 were encapsulated in 1.2% sodium alginate solution at 1x10 5 cells/ml cultured 10 beads/ well 6-well plates. The stimulated for 20 min each day using generator, effects evaluated by measuring DNA PG Furthermore, mRNA expression was analyzed cDNA microarray total RNA extracted from cells. Our no significant difference between control treated groups. production significantly upregulated HNPSV intensities 15, 30, 60, 120 mW/cm 2 compared control. results array showed that gene growth factors their receptors (BMP2, FGF7, TGFβR1 EGFRF1, VEGF). These findings suggest upregulates enhancement several matrix-related genes including factor-related genes. Safe non-invasive may be useful treatment delaying progression degeneration.