Database establishing method suitable for plant transposase accessible chromatin analysis

摘要: The invention discloses a database establishing method suitable for plant transposase accessible chromatin analysis. comprises following steps: firstly, tender tissue is subjected to pre-treatment, enzymatic hydrolysis cell walls carried out, filtering DAPI dyeing adopted sort and collect nucleuses on flow cytometer, the obtainednucleuses are filtered so as reduce interference of subcellular organelles experiment data; Tn5 digestion reaction purification qPCR determine circulating number needed by establishing; at last obtained single library equimolar mixing. According method, high quality computer via optimized two circles magnetic sorting certain ratio, that important reference provided study scientific research personnel onplant nucleus flux sequencing.