Method for sorting dividing cell by adopting flow cytometry
作者： Miaomiao Li , Tao Yao , Fei Li , Yong Hu , Sulan Bai
DOI:
关键词: Dividing cell 、 Protoplast 、 Propidium iodide 、 Microscope 、 RNA 、 Sorted Cells 、 Confocal 、 Flow cytometry 、 Biological system
摘要: The invention discloses a method for sorting dividing cell by adopting flow cytometry, and belongs to the technical field of biology. comprises following steps: dyeing an arabidopsis seedling use 10 mu g/mL propidium iodide prepare water encapsulation sheet; observing virtue laser scanning confocal microscope Leica SP2 determine position protoplast; preparing apical cells utilizing FASC (fluorescenceactivated sorter); finally extracting total RNA (ribonucleic acid). provided can be used collecting more in stage short time, so that experiment efficiency is remarkably improved. Compared with traditional separating method, acutely cells. extracted sorted has advantages high completeness quality, reliable studying subsequent gene expression profile.
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