Acute Lymphoblastic Leukemia Cells Inhibit the Differentiation of Bone Mesenchymal Stem Cells into Osteoblasts In Vitro by Activating Notch Signaling
作者： Gui-Cun Yang , You-Hua Xu , Hong-Xia Chen , Xiao-Jing Wang
DOI: 10.1155/2015/162410
关键词:
摘要: The disruption of normal hematopoiesis has been observed in leukemia, but the mechanism is unclear. Osteoblasts originate from bone mesenchymal stem cells (BMSCs) and can maintain hematopoiesis. To investigate how leukemic inhibit osteogenic differentiation BMSCs role Notch signaling this process, we cocultured with acute lymphoblastic leukemia (ALL) induction medium. expression levels Notch1, Hes1, markers Runx2, Osteopontin (OPN), Osteocalcin (OCN) were assessed by real-time RT-PCR western blotting on day 3. Alkaline phosphatase (ALP) activity was analyzed using an ALP kit, mineralization deposits detected Alizarin red S staining 14. And then treated Jagged1 anti-Jagged1 neutralizing Ab. abovementioned measured. Inhibition OPN, OCN reduction ALL cells, while signal inhibiting rescued these effects. All results indicated that could activating signaling, resulting a decreased number osteoblastic which may impair
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