Do combined nanoscale polystyrene and tetracycline impact on the incidence of resistance genes and microbial community disturbance in Enchytraeus crypticus

摘要: It has been proved that nanoplastics can effectively adsorb pollutants and thus influence their behavior availability. The combined toxic effects of nanoplastic its adsorbed pollutant on the soil fauna are still not well known. We used high-throughput quantitative PCR to explore oral nanoscale polystyrene tetracycline exposure antibiotic resistance genes in invertebrate Enchytraeus crypticus, bacterial 16S rRNA gene amplification sequencing examine response microbiome E. crypticus. After 14 days exposure, we terminated monitored restoration ARGs Results showed number ARGs, especially macrolide-lincosamide-streptogramin B (MLSB), as multidrug increased with tetracycline. abundance Aminoglycoside Beta_Lactamase crypticus also significantly increased. perturbed families Microbacteriaceae, Streptococcaceae, Enterobacteriaceae, Rhodocyclaceae Sphinomonadaceae. terminating for days, diversity were completely restored, while was permanently changed but reversibly impacted.